Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 µg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

High Selectivity for higher purity Convenient and Ready-to-Use Consistent protein binding each use Compatible with CelLytic^™ cell lysis reagents

General description

Kit contains 3 collection tubes for every spin column.

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

CelLytic is a trademark of Sigma-Aldrich Co. LLC